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Application

FC : Flow Cytometry, Intracellular Staining/Flow Cytometry

ELISA : ELISA, ELISPOT, Multiplexing Immunoassays

ICC : Immunocytochemistry

IHC : Immunohistochemistry, Immunofluorescence, Microscopy, Imaging, In Vivo Imaging

FA : Functional Assays, Bioassays, Neutralization, Depletion Studies, Biomolecule Conjugation

WB : Immunoprecipitation, Western Blotting

Pig IL-18 Platinum ELISA

製品情報

host:  
種交差: Pig 
標識: Biotin 
Interleukin-18, Interferon-gamma-inducing factor 
価格
Cat. No. Size Price キャンペーン価格
BMS672TEN 10x 96 tests ¥531,600  ¥318,960   

Description

The pig IL-18 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of pig IL-18. The pig IL-18 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acid sequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.

Details

Reactivity Pig
Sample Volume 50 uL
Suitable Sample Types cell culture supernatant, serum
Sensitivity 23.0 pg/mL
Standard Curve Range 39.1 - 2,500 pg/mL
Components Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to pig IL-18
Biotin-Conjugate anti-pig IL-18 monoclonal antibody
Streptavidin-HRP
Pig IL-18 Standard lyophilized, 5 ng/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Reported Applications ELISA

Documentation

TDS Link Download TDS

Additional Formats

References

Citations: Toka,Felix N.; Nfon,Charles K.; Dawson,Harry; Golde,William T.. Accessory cell mediated activation of porcine NK cells by TLR7 and TLR8 agonists. Clinical and Vaccine Immunology 2009;CVI(Link)

References: Takubo,T.; Ohkura,H.; Kumura,T.; Ohta,K.; Yamane,T.; Hino,M.; Kamitani,T.; Tatsumi,N.. Serum IL-18 levels in patients with various hematological disorders. Haematologia (Budap.) 2000;30:31-33. (Link)

Nakamura,H.; Komatsu,K.; Ayaki,M.; Kawamoto,S.; Murakami,M.; Uoshima,N.; Yagi,T.; Hasegawa,T.; Yasumi,M.; Karasuno,T.; Teshima,H.; Hiraoka,A.; Masaoka,T.. Serum levels of soluble IL-2 receptor, IL-12, IL-18, and IFN-gamma in patients with acute graft-versus-host disease after allogeneic bone marrow transplantation. J.Allergy Clin.Immunol. 2000;106:S45-S50. (Link)

Companjen,A.R.; van,der Velden,V; Vooys,A.; Debets,R.; Benner,R.; Prens,E.P.. Human keratinocytes are major producers of IL-18: predominant expression of the unprocessed form 31. Eur.Cytokine Netw. 2000;11:383-390. (Link)

Dinarello,C.A.; Novick,D.; Puren,A.J.; Fantuzzi,G.; Shapiro,L.; Muhl,H.; Yoon,D.Y.; Reznikov,L.L.; Kim,S.H.; Rubinstein,M.. Overview of interleukin-18: more than an interferon-gamma inducing factor. J.Leukoc.Biol. 1998;63:658-664. (Link)

Golab,J.. Interleukin 18--interferon gamma inducing factor--a novel player in tumour immunotherapy? 46. Cytokine 2000;12:332-338. (Link)

Torre,D.; Speranza,F.; Martegani,R.; Pugliese,A.; Castelli,F.; Basilico,C.; Biondi,G.. Circulating levels of IL-18 in adult and paediatric patients with HIV-1 infection. AIDS 2000;14:2211-2212. (Link)

Tsutsui,H.; Matsui,K.; Okamura,H.; Nakanishi,K.. Pathophysiological roles of interleukin-18 in inflammatory liver diseases. Immunol.Rev. 2000;174:192-209. (Link)

Lebel-Binay,S.; Berger,A.; Zinzindohoue,F.; Cugnenc,P.; Thiounn,N.; Fridman,W.H.; Pages,F.. Interleukin-18: biological properties and clinical implications. Eur.Cytokine Netw. 2000;11:15-26. (Link)

Fantuzzi,G.; Dinarello,C.A.. Interleukin-18 and interleukin-1 beta: two cytokine substrates for ICE (caspase-1). J.Clin.Immunol. 1999;19:1-11. (Link)

Gillespie,M.T.; Horwood,N.J.. Interleukin-18: perspectives on the newest interleukin. Cytokine Growth Factor Rev. 1998;9:109-116. (Link)

Xu,D.; Trajkovic,V.; Hunter,D.; Leung,B.P.; Schulz,K.; Gracie,J.A.; McInnes,I.B.; Liew,F.Y.. IL-18 induces the differentiation of Th1 or Th2 cells depending upon cytokine milieu and genetic background. Eur.J.Immunol. 2000;30:3147-3156. (Link)

Ida,A.; Tsuji,Y.; Muranaka,J.; Kanazawa,R.; Nakata,Y.; Adachi,S.; Okamura,H.; Koyama,K.. IL-18 in pregnancy; the elevation of IL-18 in maternal peripheral blood during labour and complicated pregnancies.. J.Reprod.Immunol. 2000;47:65-74. (Link)

Grobmyer,S.R.; Lin,E.; Lowry,S.F.; Rivadeneira,D.E.; Potter,S.; Barie,P.S.; Nathan,C.F.. Elevation of IL-18 in human sepsis. J.Clin.Immunol. 2000;20:212-215. (Link)

Gracie,J.A.; Forsey,R.J.; Chan,W.L.; Gilmour,A.; Leung,B.P.; Greer,M.R.; Kennedy,K.; Carter,R.; Wei,X.Q.; Xu,D.; Field,M.; Foulis,A.; Liew,F.Y.; McInnes,I.B.. A proinflammatory role for IL-18 in rheumatoid arthritis. J.Clin.Invest 1999;104:1393-1401. (Link)

Takubo,T.; Okura,H.; Kumura,T.; Nishiki,S.; Kinoshita,Y.; Koh,K.; Ohta,K.; Yamane,T.; Hino,M.; Kamitani,T.; Tatsumi,N.. Human IL-18 bioactivity in hematological malignancies with highly elevated serum IL-18 levels. Acta Haematol. 2000;103:162-164. (Link)

Pizarro,T.T.; Michie,M.H.; Bentz,M.; Woraratanadharm,J.; Smith,M.F.,Jr.; Foley,E.; Moskaluk,C.A.; Bickston,S.J.; Cominelli,F.. IL-18, a novel immunoregulatory cytokine, is up-regulated in Crohn's disease: expression and localization in intestinal mucosal cells. J.Immunol. 1999;162:6829-6835. (Link)

Monteleone,G.; Trapasso,F.; Parrello,T.; Biancone,L.; Stella,A.; Iuliano,R.; Luzza,F.; Fusco,A.; Pallone,F.. Bioactive IL-18 expression is up-regulated in Crohn's disease. J.Immunol. 1999;163:143-147. (Link)

Endo,S.; Inada,K.; Yamada,Y.; Wakabayashi,G.; Ishikura,H.; Tanaka,T.; Sato,S.. Interleukin 18 (IL-18) levels in patients with sepsis 37. J.Med. 2000;31:15-20. (Link)

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